Welcome to In-Silicon PCR Webtool

Target Template Database:
Primer Pair Filename:

Or paste primer pair(s) into the following textfield:
[Format: PrimerID Forward_Primer(5'-3') Reserver_Primer(5'-3'). Demo]

tileSize * the size of match that triggers an alignment.
stepSize * spacing between tiles.
maxSize * Maximum size of PCR product
minPerfect * Minimum size of perfect match at 3' end of primer
minGood * Minimum size where there must be 2 matches for each mismatch
Output format